 |
NSW Arbovirus Surveillance & Vector Monitoring Program |  |
|
NSW Arbovirus Surveillance & Vector Monitoring Program | |
 1998 - 1999
Annual Report  |
Methods. Mosquitoes
were sorted into pools of 25 mosquitoes, according to locality, site, date of capture,
species, sex and (for females) whether blood-engorged or not. Each mosquito pool was then
ground (Doggett et al., 1997), centrifuged and
the supernatant inoculated onto various cell lines according to the accompanying diagram.
The C6/36
plates from the first inoculation were screened for the presence of flaviviruses via a
'fixed cell' enzyme linked immunosorbent assay (ELISA)(Broom et al. 1998). This assay is
also used to identify any suspected virus isolate, and can identify the alphaviruses - Barmah Forest (BF), RR, SIN
and Getah (GET), and the flaviviruses MVE, KUN, Alfuy (ALF), EH,
KOK and STR. Any isolate that was not identified by this assay
is labelled as 'unknown'. Note that more detailed methodology can be obtained on the NSW
Arbovirus Surveillance web site at:
<http://www.arbovirus.health.nsw.gov.au/arbovirus
/about/methods.htm>
All positive virus results were sent to Dr Jeremy McAnulty of the AIDS and Infectious Disease Branch, NSW Health and to the relevant Public Health Unit. Virus results were also posted onto the NSW Arbovirus Surveillance Web Site at:
<http://www.arbovirus.health.nsw.gov.au/arbovirus/results/virusisolates.htm> and on the
web site under each location's results.
Results.
Of
the 277,167 mosquitoes
processed, there were 126 virus isolates. These are listed in the tables below.
Inland
Arbovirus Isolates, 1998-99.
LOCATION
- Site |
Date
Trapped |
Mosquito
Species |
VIRUS
ISOLATES |
Total |
|||||
RR |
SIN |
EH |
KUN |
KOK |
? |
||||
GRIFFITH
- Barren Box |
21.12.98 |
An.
annulipes |
|
1 |
|
|
|
|
1 |
GRIFFITH
- Barren Box |
4.1.99 |
Cx.
annulirostris |
|
1 |
|
|
|
|
1 |
GRIFFITH
- Barren Box |
4.1.99 |
An.
annulipes |
|
|
|
|
|
1 |
1 |
GRIFFITH
- Barren Box |
15.2.99 |
Cx.
annulirostris |
|
2 |
|
|
|
|
2 |
GRIFFITH
- Barren Box |
22.2.99 |
Cx.
annulirostris |
|
1 |
|
|
|
|
1 |
GRIFFITH
- Hanwood |
16.11.98 |
Cx.
annulirostris |
1 |
|
|
|
|
|
1 |
GRIFFITH
- Hanwood |
16.11.98 |
An.
annulipes |
1 |
|
|
|
|
|
1 |
GRIFFITH
- Hanwood |
21.12.98 |
Cx.
annulirostris |
|
1 |
|
|
|
|
1 |
GRIFFITH
- Hanwood |
28.12.98 |
Cx.
annulirostris |
|
4 |
|
|
|
|
4 |
GRIFFITH
- Hanwood |
4.1.99 |
Cx.
annulirostris |
|
4 |
|
|
|
2 |
6 |
GRIFFITH
- Hanwood |
11.1.99 |
Cx.
annulirostris |
|
1 |
|
|
|
|
1 |
GRIFFITH
- Hanwood |
5.4.99 |
Cx.
annulirostris |
|
|
|
|
|
1 |
1 |
GRIFFITH
- Willbriggie |
2.11.98 |
Cx.
annulirostris |
1 |
|
|
|
|
|
1 |
GRIFFITH
- Willbriggie |
16.11.98 |
Cx.
annulirostris |
|
|
|
|
|
1 |
1 |
GRIFFITH
- Willbriggie |
7.12.98 |
Cx.
annulirostris |
|
|
|
|
|
1 |
1 |
GRIFFITH
- Willbriggie |
14.12.98 |
An.
annulipes |
|
|
|
|
|
1 |
1 |
GRIFFITH
- Willbriggie |
11.1.99 |
Cx.
annulirostris |
|
2 |
|
|
|
|
2 |
GRIFFITH
- Willbriggie |
11.1.99 |
An.
annulipes |
|
1 |
|
|
|
1 |
2 |
GRIFFITH
- Willbriggie |
18.1.99 |
Cx.
annulirostris |
|
1 |
|
|
|
|
1 |
GRIFFITH
- Willbriggie |
18.1.99 |
An.
annulipes |
|
|
|
|
|
3 |
3 |
GRIFFITH
- Willbriggie |
1.2.99 |
Cx.
annulirostris |
|
|
1 |
|
1 |
|
2 |
GRIFFITH
- Willbriggie |
15.2.99 |
Cx.
annulirostris |
|
|
|
1 |
|
|
1 |
GRIFFITH
- Willbriggie |
1.3.99 |
Cx.
annulirostris |
|
||||||